Jota was substituted off in the 30th minute due to an injury and Darwin Nunez came on to replace him.
Slot said, “Diogo had to be substituted and I am not sure what it is, but I’d be surprised if he is there on Wednesday.”
Liverpool won 2-1 on Sunday and meet RB Leipzig in the Champions League on Wednesday.
The Reds are currently top of the Premier League table.